Rabbit anti-TIF1 Alpha/TRIM24 Antibody

SKU: orb1529583

Description

TIF1 Alpha/TRIM24 Antibody

Images & Validation

• Tested Applications: ChIP, IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 700 and 750
• Target: TIF1 Alpha/TRIM24
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

TF1A; TIF1; Tripartite motif-containing protein 24; transcriptional intermediary factor 1; transcription intermediary factor 1-alpha; TIF1-alpha; TIF1ALPHA; E3 ubiquitin-protein ligase TRIM24; RNF82; RING-type E3 ubiquitin transferase TIF1-alpha; RING finger protein 82; PTC6; hTIF1; TIF1A

Detection of human TIF1A by western blot.Samples: Whole cell lysate from K-562, HEK293T, HeLa, MCF-7, and Hep-G2 cells. Antibody: Affinity purified rabbit anti-TIF1A antibody at 0.1 µg/ml.

Detection of mouse TIF1A by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-TIF1A antibody at 0.1 µg/ml.

Detection of human TIF1 alpha/TRIM24 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TIF1 alpha/TRIM24 used at a dilution of 1:200 (1µg/ml).

Detection of mouse TIF1 alpha/TRIM24 by immunohistochemistry. Sample: FFPE section of mouse intestine. Antibody: Affinity purified rabbit anti-TIF1 alpha/TRIM24 used at a dilution of 1:200 (1µg/ml).

Detection of human TIF1A by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-TIF1A antibody.

Localization of TIF1 Alpha/TRIM24 Binding Sites by ChIP-sequencing.Chromatin from Breast cancer cell line T47D was immunoprecipitated with anti-TIF1 Alpha/TRIM24 antibody and analyzed by DNA sequencing.

Quick Database Links

Gene Symbol

TIF1 Alpha/TRIM24

UniProt

O15164

RefSeq (Protein):NP_056989.2