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Rabbit THOC5 Antibody
Description
Images & Validation
−| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 25 and 75 |
| Target | THOC5 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 200 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−THOC5 Rabbit Polyclonal Antibody [orb631876]
ELISA, IF, IHC, IP, WB
Human, Mouse, Rat
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50 μg, 100 μgTHOC5 Rabbit Polyclonal Antibody [orb1474673]
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Human, Mouse
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200 μl, 100 μl, 30 μl, 50 μlRabbit THOC5 Antibody [orb1527180]
IP, WB
Bovine, Frog, Gallus, Mouse, Xenopus
Human
Rabbit
Polyclonal
Unconjugated
100 μl, 10 μl

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Detection of human and mouse THOC5 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50µg) cells. Antibodies: Affinity purified rabbit anti-THOC5 antibody orb1527182 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. THOC5 was also immunoprecipitated by rabbit anti-THOC5 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human THOC5 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-THOC5 (Cat. No. orb1527182) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_003669.4 |
|---|
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Protocol Information
Rabbit THOC5 Antibody (orb1527182)
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