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| Catalog Number | orb1527182 |
|---|---|
| Category | Antibodies |
| Description | THOC5 Antibody |
| Target | THOC5 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Form/Appearance | Whole IgG |
| Concentration | 200 µg/ml |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Immunogen | Between 25 and 75 |
| UniProt ID | Q13769 |
| Tested applications | IHC, IP, WB |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Application notes | Format: Whole IgG |
| Antibody Type | Primary Antibody |
| Storage | 2 - 8°C |
| Alternative names | C22orf19; Fmip; Fms-interacting protein; fSAP79; f Read more... |
| Note | For research use only |
| NCBI | NP_003669.4 |
| Expiration Date | 12 months from date of receipt. |

Detection of human and mouse THOC5 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50µg) cells. Antibodies: Affinity purified rabbit anti-THOC5 antibody orb1527182 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. THOC5 was also immunoprecipitated by rabbit anti-THOC5 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human THOC5 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-THOC5 (Cat. No. orb1527182) used at a dilution of 1: 200 (1µg/ml). Detection: DAB
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