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Rabbit anti-TGOLN2 Antibody
Catalog Number: orb1523001
| Catalog Number | orb1523001 |
|---|---|
| Category | Antibodies |
| Description | TGOLN2 Antibody |
| Target | TGOLN2 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Whole IgG |
| Concentration | 1000 µg/ml |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Immunogen | Between 175 and 225 |
| UniProt ID | O43493 |
| Tested applications | IHC, IP, WB |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Application notes | Format: Whole IgG |
| Antibody Type | Primary Antibody |
| Storage | 2 - 8°C |
| Alternative names | trans-Golgi network protein TGN51; trans-Golgi net Read more... |
| Note | For research use only |
| NCBI | NP_001193769.1 |
Detection of human TGOLN2 by western blot. Samples: Whole cell lysate (50 µg) prepared using NETN buffer from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TGOLN2 antibody orb1523001 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human TGOLN2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-TGOLN2 antibody orb1523001 used for IP at 6 µg per reaction.
Detection of human TGOLN2 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TGOLN2 (Cat. No. orb1523001) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
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