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Rabbit TCEB2 Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:500 - 1:2,500; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 68 and C-terminus |
| Target | TCEB2 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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100 μl

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Detection of human and mouse TCEB2 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, HeLa, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-TCEB2 antibody orb1523763 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human TCEB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-TCEB2 antibody orb1523763 used for IP at 6 µg/mg lysate.

Detection of human TCEB2 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-TCEB2 (Cat. No. orb1523763) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of mouse TCEB2 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-TCEB2 (Cat. No. orb1523763) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_009039.1 |
|---|
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Protocol Information
Rabbit TCEB2 Antibody (orb1523763)
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