Rabbit TBXT Antibody

SKU: orb1520241

Description

TBXT Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 15-20 µl/mg lysate
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 385 and C-terminus
• Target: TBXT
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

brachyury protein; protein T; SAVA; T; T brachyury transcription factor; T, brachyury homolog; T-box transcription factor T; TFT

Detection of human TBXT by western blot. Samples: Whole cell lysate (50 µg) from NCI-H460, SR, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TBXT antibody (orb1520241) used for WB at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human TBXT by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NCI-H460 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TBXT antibody (orb1520241) used for IP at 6 µg per reaction.

Quick Database Links

Gene Symbol

TBXT

UniProt

O15178

RefSeq (Protein):NP_003172.1