Rabbit TAF5 Antibody

SKU: orb1524377

Description

TAF5 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 175 and 225
• Target: TAF5
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TAF(II)100; TAF2D; TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa; TAFII100; TAFII-100; TATA box binding protein (TBP)-associated factor 2D; TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD; transcription initiation factor TFIID 100 kD subunit; transcription initiation factor TFIID 100 kDa subunit; transcription initiation factor TFIID subunit 5

Detection of human TAF5 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TAF5 antibody orb1524377 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. TAF5 was also immunoprecipitated by rabbit anti-TAF5 antibodies, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human TAF5 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-TAF5 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

TAF5

UniProt

Q15542

RefSeq (Protein):NP_008882.2