Rabbit anti-SOX10 Recombinant Monoclonal Antibody

• Catalog Number: orb1519802
• Category: Antibodies
• Description: SOX10 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR080G
• Tested applications: ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 416 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 50 µg/ml
• Dilution range: (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 20 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Form/Appearance: Whole IgG
• Purity: Recombinant antibody was purified from cell culture supernatant
• Conjugation: Unconjugated
• Target: SOX10
• UniProt ID: P56693
• NCBI: NP_008872.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: transcription factor SOX-10; SRY-related HMG-box g
• Background: SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. SOX10 may act as a transcriptional activator after forming a protein complex with other proteins. SOX10 acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development
• Note: For research use only
• Application notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
• Expiration Date: 12 months from date of receipt.

Detection of human SOX10 in FFPE SK-MEL-28 cells by ICC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (orb1519802). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human SOX10 by western blot. Samples: Whole cell lysate (15 µg) from HEK293T, Malme-3M, MCF-7, SK-MEL-28, Jurkat, MDA-MB-435, HeLa, and UACC-62 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-GAPDH

Detection of human SOX10 in FFPE melanoma by IHC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB

Detection of human SOX10 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB