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Description
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB - 1:1,000 - 1:5,000; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Predicted Reactivity | Drosophila, Mouse, Xenopus |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1 and 50 |
| Target | Sm-D3 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human Sm-D3 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) , HEK293T (50µg) , and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-Sm-D3 antibody orb1523871 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Sm-D3 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Sm-D3 (Cat. No. orb1523871) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_004166.1 |
|---|
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Protocol Information
Rabbit Sm-D3 Antibody (orb1523871)
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![PRMT7 Rabbit mAb [KD Validated] Antibody](/images/pub/media/catalog/product/NewWebsite/18/orb1950317_1.png)
![PRMT7 Rabbit mAb [KD Validated] Antibody](/images/pub/media/catalog/product/NewWebsite/18/orb1950317_2.png)
