Rabbit SHMT1 Antibody

SKU: orb1523328

Description

SHMT1 Antibody

Research Area

Cancer Biology, Metabolism Research

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:500 - 1:2,500; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 433 and C-terminus
• Target: SHMT1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CSHMT; cytoplasmic serine hydroxymethyltransferase; glycine hydroxymethyltransferase; serine hydroxymethyltransferase 1 (soluble); serine hydroxymethyltransferase, cytosolic; serine methylase; SHMT

Detection of human SHMT1 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody orb1523328 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of mouse SHMT1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody orb1523328 used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human SHMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SHMT1 antibody orb1523328 used for IP at 6 µg per reaction.

Detection of human SHMT1 by immunohistochemistry.Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-SHMT1 (Cat. No. orb1523328) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of mouse SHMT1 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-SHMT1 (Cat. No. orb1523328) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

SHMT1

UniProt

P34896

RefSeq (Protein):NP_004160.3