Rabbit Sheep IgM (heavy and light chains), conjugated with FITC Antibody

• Catalog Number: orb21555
• Category: Antibodies
• Description: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to sheep IgM, heavy and light chains. To identify IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, where IgM and IgG antibodies can be expected. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: FITC
• Reactivity: Sheep
• Form/Appearance: FITC-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added
• RRID: AB_10925520
• Antibody Type: Secondary Antibody
• Source: Purified normal IgM isolated from pooled sheep serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Storage: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
• Research Area: Stem Cells
• Note: For research use only
• Expiration Date: 12 months from date of receipt.