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Rabbit anti-SHANK3 Antibody
Catalog Number: orb1523478
| Catalog Number | orb1523478 |
|---|---|
| Category | Antibodies |
| Description | SHANK3 Antibody |
| Target | SHANK3 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Form/Appearance | Whole IgG |
| Concentration | 1000 µg/ml |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Immunogen | Between 1475 and 1525 |
| UniProt ID | Q9BYB0 |
| Tested applications | IP, WB |
| Dilution range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate |
| Application notes | Format: Whole IgG |
| Antibody Type | Primary Antibody |
| Storage | 2 - 8°C |
| Alternative names | SH3 and multiple ankyrin repeat domains protein 3; Read more... |
| Note | For research use only |
| NCBI | NP_277052.1 |
Detection of mouse SHANK3 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SHANK3 antibody (orb1523478) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human SHANK3 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, MCF-7, SR, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SHANK3 antibody (orb1523478) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human SHANK3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHANK3 antibody (orb1523478) used for IP at 6 µg per reaction.
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