Rabbit RuvBL2 Antibody

SKU: orb1526454

Description

RuvBL2 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 5 - 15 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1 and 50
• Target: RuvBL2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; CGI-46; ECP51; ECP-51; erythrocyte cytosolic protein, 51-KD; INO80 complex subunit J; INO80J; repressing pontin 52; REPTIN; Reptin 52; reptin52 protein; RuvB (E coli homolog)-like 2; ruvB-like 2; RVB2; TAP54-beta; TIH2; TIP48; TIP49B; TIP60-associated protein 54-beta

Detection of human and mouse RuvBL2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RuvBL2 antibody orb1526454 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. RuvBL2 was also immunoprecipitated by rabbit anti-RuvBL2 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human RuvBL2 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RuvBL2 (Cat. No. orb1526454) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

RuvBL2

UniProt

Q9Y230

RefSeq (Protein):NP_006657.1