Rabbit RPC2 Antibody

SKU: orb1527673

Description

RPC2 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1083 and C-terminus
• Target: RPC2
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C128; DNA-directed RNA polymerase III 127.6 kDa polypeptide; DNA-directed RNA polymerase III subunit B; DNA-directed RNA polymerase III subunit RPC2; HLD8; INMAP; interphase nucleus and mitotic apparatus associated protein; polymerase (RNA) III (DNA directed) polypeptide B; polymerase (RNA) III subunit B; RNA polymerase III subunit C2; RPC2

Detection of human RPC2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPC2 antibody orb1527673 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human RPC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPC2 antibody orb1527673 used for IP at 3 µg per reaction.

Detection of human RPC2 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RPC2 (Cat. No. orb1527673) used at a dilution of 1: 200 (1 µg/ml). Detection: DAB

Detection of human RPC2 by immunohistochemistry.Sample: FFPE section of human stomach linitis plastica cancer. Antibody: Affinity purified rabbit anti-RPC2 (Cat. No. orb1527673) used at a dilution of 1: 200 (1 µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

RPC2

UniProt

Q9NW08

RefSeq (Protein):NP_060552.3