Rabbit anti-RPC2 Antibody

• Catalog Number: orb1527673
• Category: Antibodies
• Description: RPC2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 1083 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RPC2
• UniProt ID: Q9NW08
• NCBI: NP_060552.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: interphase nucleus and mitotic apparatus associate
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human RPC2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPC2 antibody orb1527673 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human RPC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPC2 antibody orb1527673 used for IP at 3 µg per reaction.

Detection of human RPC2 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-RPC2 (Cat. No. orb1527673) used at a dilution of 1: 200 (1 µg/ml). Detection: DAB

Detection of human RPC2 by immunohistochemistry.Sample: FFPE section of human stomach linitis plastica cancer. Antibody: Affinity purified rabbit anti-RPC2 (Cat. No. orb1527673) used at a dilution of 1: 200 (1 µg/ml). Detection: DAB