Rabbit RNF20 Antibody

SKU: orb1529747

Description

RNF20 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Gallus
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 125 and 175
• Target: RNF20
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BRE1; BRE1 E3 ubiquitin ligase homolog; BRE1A; BRE1-A; E3 ubiquitin-protein ligase BRE1A; hBRE1; homolog of S. cerevisiae BRE1; RING finger protein 20; RING-type E3 ubiquitin transferase BRE1A

Detection of human RNF20 by western blot.Samples: Whole cell lysate from HeLa and HEK293T cells. Antibody: Affinity purified rabbit anti-RNF20 antibody orb1529747 at 0.1 µg/ml.

Detection of human RNF20 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RNF20 used at a dilution of 1:1, 000 (1µg/ml).

Detection of mouse RNF20 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-RNF20 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human RNF20 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-RNF20 antibody.

Quick Database Links

Gene Symbol

RNF20

UniProt

Q5VTR2

RefSeq (Protein):NP_062538.5