Rabbit Reticulon-3 Antibody

SKU: orb1525863

Description

Reticulon-3 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 750 and 800
• Target: Reticulon-3
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ASY interacting protein; ASYIP; HAP; homolog of ASY protein; neuroendocrine-specific protein-like 2; neuroendocrine-specific protein-like II; NSPL2; NSPLII; NSP-like protein 2; NSP-like protein II; reticulon-3; RTN3-A1

Detection of human Reticulon-3 by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-Reticulon-3 antibody orb1525863 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Reticulon-3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Reticulon-3 antibody orb1525863 used for IP at 3 µg/mg lysate.

Detection of human Reticulon-3 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Reticulon-3 (Cat. No. orb1525863) used at a dilution of 1: 200 (1µg/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate

Quick Database Links

Gene Symbol

Reticulon-3

UniProt

O95197

RefSeq (Protein):NP_006045.1