Rabbit anti-Reticulon-3 Antibody

• Catalog Number: orb1525863
• Category: Antibodies
• Description: Reticulon-3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 750 and 800
• Antibody Type: Primary Antibody
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Reticulon-3
• UniProt ID: O95197
• NCBI: AAU81930.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: NSPL2; reticulon-3; NSP-like protein II; RTN3-A1;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Reticulon-3 by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-Reticulon-3 antibody orb1525863 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Reticulon-3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Reticulon-3 antibody orb1525863 used for IP at 3 µg/mg lysate.

Detection of human Reticulon-3 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Reticulon-3 (Cat. No. orb1525863) used at a dilution of 1: 200 (1µg/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate