Rabbit RENT1 Antibody

SKU: orb1527586

Description

RENT1 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1079 and C-terminus
• Target: RENT1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

ATP-dependent helicase RENT1; delta helicase; HUPF1; nonsense mRNA reducing factor 1; NORF1; pNORF1; regulator of nonsense transcripts 1; RENT1; smg-2; smg-2 homolog, nonsense mediated mRNA decay factor; UPF1 regulator of nonsense transcripts homolog; up-frameshift mutation 1 homolog; up-frameshift suppressor 1 homolog; yeast Upf1p homolog

Detection of mouse RENT1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RENT1 antibody (orb1527586) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human RENT1 by western blot. Samples: Whole cell lysate (50 µg) from K-562, HEK293T, MCF-7, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RENT1 antibody (orb1527586) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human RENT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RENT1 antibody (orb1527586) used for IP at 4 µg per reaction. RENT1 was also immunoprecipitated by a previous lot of this antibody (orb1527586) and a second antibody against a different epitope of RENT1.

Quick Database Links

Gene Symbol

RENT1

UniProt

Q92900

RefSeq (Protein):NP_002902.2