Rabbit anti-RBM22 Antibody

• Catalog Number: orb1523927
• Category: Antibodies
• Description: RBM22 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 175 and 225
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RBM22
• UniProt ID: Q9NW64
• NCBI: NP_060517.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Cwc2; zinc finger CCCH domain-containing protein 1
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse RBM22 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM22 antibody (orb1523927) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human RBM22 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, Jurkat, OVCAR-8, and SR cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RBM22 antibody (orb1523927) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human RBM22 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RBM22 antibody (orb1523927) used for IP at 6 µg per reaction. RBM22 was also immunoprecipitated by a previous lot of this antibody (orb1523927) and a second antibody against a different epitope of RBM22.