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Catalog Number | orb700243 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody against Rabbit anti-Rat IgG (H&L) conjugated to HRP |
Species/Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Purified rat IgG (H&L) |
Dilution range | This conjugate is suitable for all immunoassay applications, The optimal working dilution should be determined by the investigator |
Form/Appearance | Lyophilized |
Purity | Immunogen affinity purified rabbit IgG. |
Conjugation | HRP |
Storage | Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. |
Note | For research use only |
Application notes | Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rat IgG, light chains on all rat immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rat serum proteins. |
Expiration Date | 12 months from date of receipt. |
ELISA, IHC-Fr, IHC-P, WB | |
Rat | |
Rabbit | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Rat | |
Rabbit | |
Polyclonal | |
HRP |
ELISA, IH, WB | |
Rat | |
Rabbit | |
Polyclonal | |
HRP |
ELISA, IHC, WB | |
Rat | |
Rabbit | |
Polyclonal | |
HRP |