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Rabbit anti-Pygo1 Antibody
Catalog Number: orb1526252
| Catalog Number | orb1526252 |
|---|---|
| Category | Antibodies |
| Description | Pygo1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, IP |
| Reactivity | Human |
| Immunogen | Between 300 and 350 |
| Antibody Type | Primary Antibody |
| Concentration | 1000 µg/ml |
| Dilution range | WB - Not recommended. Use rabbit anti-Pygo1 antibody A302-649A.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | Pygo1 |
| UniProt ID | Q9Y3Y4 |
| NCBI | NP_056432.1 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Alternative names | pygopus-like protein 1; pygopus homolog 1 Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human Pygo1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Pygo1 antibody orb1526252 used for IP at 3 µg/mg lysate.
Detection of human Pygo1 by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Pygo1 (Cat. No. orb1526252) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
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