You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
Rabbit anti-PUS1 Antibody
Catalog Number: orb1528025
| Catalog Number | orb1528025 |
|---|---|
| Category | Antibodies |
| Description | PUS1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, IP, WB |
| Reactivity | Human |
| Immunogen | Between 377 and C-terminus |
| Antibody Type | Primary Antibody |
| Concentration | 200 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | PUS1 |
| UniProt ID | Q9Y606 |
| NCBI | NP_079491.2 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Alternative names | tRNA pseudouridine synthase A, mitochondrial; tRNA Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human PUS1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PUS1 antibody orb1528025 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. PUS1 was also immunoprecipitated by rabbit anti-PUS1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Detection of human PUS1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PUS1 (Cat. No. orb1528025) used at a dilution of 1: 1, 000 (0.2 µg/ml). Detection: DAB
- Item 1 of 2
- Item 1 of 1
