Rabbit PSMD3 Antibody

SKU: orb1524112

Description

PSMD3 Antibody

Research Area

Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1 and 50
• Target: PSMD3
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

26S proteasome non-ATPase regulatory subunit 3; 26S proteasome regulatory subunit RPN3; 26S proteasome regulatory subunit S3; P58; proteasome (prosome, macropain) 26S subunit, non-ATPase, 3; proteasome subunit p58; RPN3; S3; tissue specific transplantation antigen 2; TSTA2

Detection of human and mouse PSMD3 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HeLa (H; 50 µg) , Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PSMD3 antibody orb1524112 used for WB at 0.1 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. PSMD3 was also immunoprecipitated by rabbit anti-PSMD3 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human PSMD3 by immunohistochemistry. Samples: FFPE sections of human lung cancer (left) and prostate carcinoma (right). Antibody: Affinity purified rabbit anti-PSMD3 (Cat. No. orb1524112) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).

Quick Database Links

Gene Symbol

PSMD3

UniProt

O43242

RefSeq (Protein):NP_002800.2