Rabbit PSMC1 Antibody

SKU: orb1524120

Description

PSMC1 Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 390 and C-terminus
• Target: PSMC1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

26S protease regulatory subunit 4; 26S proteasome AAA-ATPase subunit RPT2; 26S proteasome regulatory subunit 4; P26S4; p56; proteasome (prosome, macropain) 26S subunit, ATPase, 1; proteasome 26S ATPase subunit 1; Proteasome 26S subunit ATPase 1; S4

Detection of human and mouse PSMC1 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HeLa (H; 50 µg) , Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PSMC1 antibody orb1524120 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. PSMC1 was not successfully immunoprecipitated by rabbit anti-PSMC1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Detection of mouse PSMC1 by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PSMC1 (Cat. No. orb1524120) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human PSMC1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PSMC1 (Cat. No. orb1524120) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

PSMC1

UniProt

P62191

RefSeq (Protein):NP_002793.2