Rabbit POLR3E Antibody

SKU: orb1524333

Description

POLR3E Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 475 and 525
• Target: POLR3E
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA-directed RNA polymerase III 80 kDa polypeptide; DNA-directed RNA polymerase III subunit RPC5; polymerase (RNA) III (DNA directed) polypeptide E (80kD); polymerase (RNA) III subunit E; RNA polymerase III 80 kDa subunit RPC5; RNA polymerase III subunit C5; RPC5; SIN

Detection of human POLR3E by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-POLR3E antibody orb1524333 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. POLR3E was also immunoprecipitated by rabbit anti-POLR3E antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Quick Database Links

Gene Symbol

POLR3E

UniProt

Q9NVU0

RefSeq (Protein):NP_060589.1