Rabbit anti-PolD1 Antibody

• Catalog Number: orb1523769
• Category: Antibodies
• Description: PolD1 Antibody
• Target: PolD1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 75 and 125
• UniProt ID: P28340
• Tested applications: IHC, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: DNA polymerase subunit delta p125; polymerase (DNA
• Note: For research use only
• NCBI: NP_002682.2

Detection of human and mouse PolD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-PolD1 antibody orb1523769 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of mouse PolD1 by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-PolD1 (Cat. No. orb1523769) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human PolD1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PolD1 (Cat. No. orb1523769) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).