Rabbit anti-Phospho SMC1 (S966) Antibody

SKU: orb1530760

Description

SMC1, Phospho (S966) Antibody

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:500 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Surrounding serine 966
• Target: SMC1, Phospho (S966)
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

SMC protein 1A; SMC-1A; structural maintenance of chromosomes protein 1A; SMCB; SMC1L1; SMC-1-alpha; SMC1alpha; SMC1; segregation of mitotic chromosomes 1; SB1.8; epididymis secretory sperm binding protein; DXS423E; CDLS2; SMC1 (structural maintenance of chromosomes 1, yeast)-like 1

Detection of Phospho SMC1 (S966) by western blot.Samples: Jurkat cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody orb1530760 used at 0.1 µg/ml.

Detection of human Phospho SMC1 (S966) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) used at a dilution of 1:1, 000 (1µg/ml).

Detection of human Phospho SMC1 (S966) by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) used at a dilution of 1:1, 000 (1µg/ml).

Detection of human Phospho SMC1 (S966) by western blot of immunoprecipitates.Samples: Whole cell lysate from Jurkat cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho SMC1 (S966) antibody.

Detection of human Phospho SMC1 (S966) by ICC.Samples: HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) used at a dilution of 1:5, 000. Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated.

Quick Database Links

Gene Symbol

SMC1, Phospho (S966)

UniProt

Q14683

RefSeq (Protein):NP_006297.2