Rabbit Phospho MEK1(T286) Antibody

SKU: orb1524513

Description

MEK1, Phospho (T286) Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: IHC, IP
• Dilution Range: WB - Not recommended; however this product has been shown to perform in western blot on immunoprecipitated MEK1; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Rabbit, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Surrounding threonine 286
• Target: MEK1 Phospho (T286)
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CFC3; dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEK1; MKK1; PRKMK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Detection of human Phospho-MEK1 (T286) by immunoprecipitation (IP). Samples: 50 µg HeLa whole cell lysate (WCL) from mock treated (-) or nocodazole treated (+) cells. 1 mg of HeLa WCL mock treated (-) or nocodazole treated (+) and immunoprecipitated; 20% of the IP was loaded. Antibodies: Affinity purified rabbit anti-phospho MEK1 (T286) orb1524513 was used for IP (6ug/1mg lysate). To detect total MEK1, Rabbit anti-MEK1 was used for western blot (WB) at 1 µg/ml.

Detection of mouse Phospho-MEK1 (T286) by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-phospho MEK1 (T286) (Cat. No. orb1524513) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human Phospho-MEK1 (T286) by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-phospho MEK1 (T286) (Cat. No. orb1524513) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

MEK1 Phospho (T286)

UniProt

Q02750

RefSeq (Protein):NP_002746.1