Rabbit PHF16 Antibody

SKU: orb1524463

Description

PHF16 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 773 and C-terminus
• Target: PHF16
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

jade family PHD finger protein 3; JADE-3; PHD finger protein 16; PHF16; protein Jade-3

Detection of human PHF16 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HeLa, HEK293T, and SW620 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PHF16 antibody (orb1524463) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human PHF16 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PHF16 antibody (orb1524463) used for IP at 6 µg per reaction. PHF16 was also immunoprecipitated by a previous lot of this antibody (orb1524463) and a second antibody against a different epitope of PHF16.

Quick Database Links

Gene Symbol

PHF16

UniProt

Q92613

RefSeq (Protein):NP_055550.1