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Description
Research Area
Images & Validation
−| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 344 and C-terminus |
| Target | PHAX |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of mouse PHAX by western blot. Samples: Whole cell lysate from mouse NIH 3T3 (15 and 50 µg) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody orb1523939 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human PHAX by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-PHAX antibody orb1523939 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human PHAX by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PHAX antibody orb1523939 used for IP at 6 µg/mg lysate.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_115553.2 |
|---|
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Protocol Information
Rabbit PHAX Antibody (orb1523939)
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