Rabbit anti-PDZ-RhoGEF Antibody

• Catalog Number: orb1527500
• Category: Antibodies
• Description: PDZ-RhoGEF Antibody
• Target: PDZ-RhoGEF
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 150 and 200
• UniProt ID: O15085
• Tested applications: IHC, IP
• Dilution range: WB - Not recommended. Use rabbit anti-PDZ-RhoGEF antibody A301-952A.; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: rho guanine nucleotide exchange factor 11; RhoA-sp
• Note: For research use only
• NCBI: NP_055599.1

Detection of human PDZ-RhoGEF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PDZ-RhoGEF antibody orb1527500 used for IP at 3 µg/mg lysate.

Detection of human PDZ-RhoGEF by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PDZ-RhoGEF (Cat. No. orb1527500) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB