Rabbit PDZ-GEF1 Antibody

SKU: orb1527470

Description

PDZ-GEF1 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 1000 and 1050
• Target: PDZ-GEF1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CNrasGEF; cyclic nucleotide ras GEF; neural RAP guanine nucleotide exchange protein; nRap GEP; NRAPGEP; PDZ domain containing guanine nucleotide exchange factor (GEF) 1; PDZ domain-containing guanine nucleotide exchange factor 1; PDZGEF1; PDZ-GEF1; RA(Ras/Rap1A-associating)-GEF; RAGEF; RA-GEF; RA-GEF-1; Rap guanine nucleotide exchange factor (GEF) 2; rap guanine nucleotide exchange factor 2; Rap-GEP; ras/Rap1-associating GEF-1

Detection of human PDZ-GEF1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, Hep-G2, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDZ-GEF1 antibody orb1527470 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human PDZ-GEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody orb1527470 used for IP at 6 µg per reaction.

Detection of human PDZ-GEF1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PDZ-GEF1 (Cat. No. orb1527470) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

PDZ-GEF1

UniProt

Q9Y4G8

RefSeq (Protein):NP_055062.1