Rabbit anti-PCNA Antibody

SKU: orb1530430

Description

PCNA Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:5,000 - 1:20,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; IHC-IF - 1:1,000 - 1:5,000
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 211 and C-terminus
• Target: PCNA

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

proliferating cell nuclear antigen; PCNA; DNA polymerase delta auxiliary protein; ATLD2; cyclin

Detection of mouse PCNA by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-PCNA antibody at 0.1 µg/ml.

Detection of human PCNA by western blot.Samples: Whole cell lysate from A-549, Jurkat, HEK293T, MCF-7, and HeLa cells. Antibody: Affinity purified rabbit anti-PCNA antibody at 0.1 µg/ml.

Detection of mouse PCNA by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-PCNA used at a dilution of 1:5000 (0.2 µg/ml).

Detection of human PCNA by immunohistochemistry. Sample: FFPE section of human breast. Antibody: Affinity purified rabbit anti-PCNA used at a dilution of 1:5000 (0.2 µg/ml).

Detection of human PCNA by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-PCNA antibody.

Detection of human PCNA by IHC. Sample: human stomach carcinoma. Antibody: Affinity purified rabbit anti-PCNA at 1:4, 000. Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated.

Quick Database Links

Gene Symbol

PCNA

UniProt

P12004

RefSeq (Protein):NP_872590.1