Rabbit anti-PBK Antibody

• Catalog Number: orb1527042
• Category: Antibodies
• Description: PBK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Immunogen: Between 200 and 250
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-PBK antibody A302-194A.; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PBK
• UniProt ID: Q96KB5
• NCBI: NP_060962.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: HEL164; cancer/testis antigen 84; SPK; CT84; T-LAK
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PBK by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PBK antibody orb1527042 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. PBK was successfully immunoprecipitated by rabbit anti-PBK antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human PBK by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PBK (Cat. No. orb1527042) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB