Rabbit PBK Antibody

SKU: orb1527042

Description

PBK Antibody

Images & Validation

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Item 1 of 2

Tested Applications	IHC, WB
Dilution Range	WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-PBK antibody A302-194A.; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Reactivity	Human
Application Notes	Format: Whole IgG

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Between 200 and 250
Target	PBK
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	2 - 8°C
Form/Appearance	Liquid
Buffer/Preservatives	Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration	1000 µg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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cancer/testis antigen 84; CT84; epididymis luminal protein 164; HEL164; lymphokine-activated killer T-cell-originated protein kinase; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; serine/threonine protein kinase; spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase; TOPK

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Detection of human PBK by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PBK antibody orb1527042 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. PBK was successfully immunoprecipitated by rabbit anti-PBK antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Detection of human PBK by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PBK (Cat. No. orb1527042) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

PBK

UniProt

Q96KB5

RefSeq (Protein):NP_060962.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_060962.2

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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