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Rabbit anti-PAM Antibody
Catalog Number: orb1527709
| Catalog Number | orb1527709 |
|---|---|
| Category | Antibodies |
| Description | PAM Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | WB |
| Reactivity | Human |
| Immunogen | Between 2475 and 2525 |
| Antibody Type | Primary Antibody |
| Concentration | 200 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended. |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | PAM |
| UniProt ID | O75592 |
| NCBI | NP_055872.3 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Alternative names | pam/highwire/rpm-1 protein; E3 ubiquitin-protein l Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human PAM by western blot and immunoprecipitation. Samples: Whole cell lysate (50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PAM antibody orb1527709 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. PAM was also immunoprecipitated by rabbit anti-PAM antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).
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