Rabbit anti-PAF Antibody

• Catalog Number: orb1523114
• Category: Antibodies
• Description: PAF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended. Use rabbit anti-PAF antibody A304-374A.; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: PAF
• UniProt ID: Q15004
• NCBI: NP_055551.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: HCV NS5A-transactivated protein 9; PCNA-clamp-asso
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human PAF by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-PAF antibody orb1523114 used for IP at 6 µg per reaction.

Detection of human PAF by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-PAF (Cat. No. orb1523114) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).