Rabbit p66alpha Antibody

SKU: orb1526764

Description

p66alpha Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 583 and C-terminus
• Target: p66alpha
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GATA zinc finger domain-containing protein 2A; Hp66alpha; p66alpha; transcriptional repressor p66-alpha

Detection of human p66alpha by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p66alpha antibody orb1526764 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human p66alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p66alpha antibody orb1526764 used for IP at 6 µg per reaction.

Detection of human p66alpha by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-p66alpha (Cat. No. orb1526764) used at a dilution of 1: 1, 000 (1 µg/ml). Detection: DAB

Detection of mouse p66alpha by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-p66alpha (Cat. No. orb1526764) used at a dilution of 1: 1, 000 (1 µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

p66alpha

UniProt

Q86YP4

RefSeq (Protein):NP_060130.3