Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody

• Catalog Number: orb1784574
• Category: Antibodies
• Description: Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR268L
• Tested applications: FC, ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Residues 42-709 (ECD)
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: Flow-Cyt Live or cells fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells. Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunoprecipitation (IP) 6 µl/mg lysate. Multiplex Immunofluorescence (mIF) 1:100. Western Blot (WB) 1:1000
• Form/Appearance: Whole IgG
• Purity: Recombinant antibody was purified from cell culture supernatant
• Conjugation: Unconjugated
• Target: P-Selectin/CD62P
• UniProt ID: Q01102
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: LECAM3; platelet activation dependent granule-exte
• Background: P-Selectin, or CD62P, is a Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. It mediates the interaction of activated endothelial cells or platelets with leukocytes
• Note: For research use only
• Application notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight
• Expiration Date: 12 months from date of receipt.

Detection of human P-Selectin/CD62P by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Platelets, Jurkat, HEL 92.1.7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse P-Selectin/CD62P by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, bEnd.3 (10 µg) , CH27, Spleen, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human P-Selectin/CD62P by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse P-Selectin/CD62P by immunohistochemistry. Sample: FFPE section of mouse ovary. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse P-Selectin/CD62P (shaded) in b.END3 cells by flow cytometry. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P (shaded) in Hel92.1.7 cells by flow cytometry. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEL 92.1.7 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574) used for IP at 6 µl/mg lysate.

Detection of mouse P-Selectin/CD62P by immunocytochemistry. Sample: FFPE section of bEnd.3 cells. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P by immunocytochemistry. Sample: FFPE section of HEL 92.1.7 cells. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P (orange) by immunofluorescence. Sample: FFPE section of metastatic lymph node. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784574) used at 1:100. Secondary: Invitrogen Goat anti-rabbit IgG Alexa Fluor™ Plus 647 1:400. Counterstain: DAPI (blue).