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Catalog Number | orb1522833 |
---|---|
Category | Antibodies |
Description | NIR Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human |
Immunogen | Between 699 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | NIR |
UniProt ID | Q9Y3T9 |
NCBI | NP_056473.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | novel INHAT (inhibitor of histone acetyltransferas Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human NIR by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NIR antibody orb1522833 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human NIR by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NIR (Cat. No. orb1522833) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |