Rabbit NF-YA Antibody

SKU: orb1527208

Description

NF-YA Antibody

Research Area

Immunology & Inflammation, Metabolism Research

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 297 and C-terminus
• Target: NF-YA
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CAAT box DNA-binding protein subunit A; CAAT-box DNA binding protein subunit A; CBF-A; CBF-B; CCAAT-binding transcription factor subunit B; HAP2; HAP2 CCAAT-binding protein; NF-YA; nuclear transcription factor Y subunit A; nuclear transcription factor Y subunit alpha; nuclear transcription factor Y, alpha; Transcription factor NF-Y, A subunit

Detection of human and mouse NF-YA by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-NF-YA antibody orb1527208 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human NF-YA by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NF-YA antibody orb1527208 used for IP at 3 µg/mg lysate.

Detection of human and mouse NF-YA by immunohistochemistry.Sample: FFPE section of human breast carcinoma (left) and mouse hybridoma tumor (right). Antibody: Affinity purified rabbit anti-NF-YA (Cat. No. orb1527208) used at a dilution of 1: 200 (1 µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

NF-YA

UniProt

P23511

RefSeq (Protein):NP_068351.1