Rabbit anti-NEDD-9/CASL/HEF1 Recombinant Monoclonal Antibody

• Catalog Number: orb1733240
• Category: Antibodies
• Description: NEDD-9/CASL/HEF1 Antibody
• Target: NEDD-9/CASL/HEF1
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Immunogen: Between 150 and 200
• UniProt ID: Q14511
• Tested applications: IP, WB
• Dilution range: WB: 1:1000, IP: 6 µl/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Clone Number: BLR248L
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CRK-associated substrate-related protein; Cas scaf
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection of human NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 µg) from OVCAR-8, U-251MG, A-549, and UACC-257 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (orb1733240) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and C2C12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (orb1733240) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human NEDD-9/CASL/HEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (orb1733240) used for IP at 6 µl/mg lysate.