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Rabbit anti-NDE1 Antibody
Catalog Number: orb1527458
| Catalog Number | orb1527458 |
|---|---|
| Category | Antibodies |
| Description | NDE1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | WB |
| Reactivity | Human |
| Immunogen | Between 75 and 125 |
| Antibody Type | Primary Antibody |
| Concentration | 200 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-NDE1 antibody A301-974A. |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | NDE1 |
| UniProt ID | Q9NXR1 |
| NCBI | NP_060138.1 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Alternative names | nuclear distribution protein nudE homolog 1; nudE Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human NDE1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NDE1 antibody orb1527458 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. NDE1 was also immunoprecipitated by rabbit anti-NDE1 antibodies, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
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