Rabbit Mre11 Antibody

SKU: orb1523783

Description

Mre11 Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 658 and C-terminus
• Target: Mre11
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ATLD; AT-like disease; DNA recombination and repair protein; double-strand break repair protein MRE11; double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A; MRE11 double strand break repair nuclease A; MRE11 homolog 1; MRE11 homolog A; MRE11 homolog A, double strand break repair nuclease; MRE11 homolog, double strand break repair nuclease A; MRE11 meiotic recombination 11 homolog A; MRE11 meiotic recombination 11-like protein A; MRE11A; MRE11B

Detection of human Mre11 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Mre11 antibody orb1523783 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Mre11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-Mre11 antibody orb1523783 used for IP at 6 µg/mg lysate.

Detection of human Mre11 by immunohistochemistry.Sample: FFPE section of human stomach linitis plastica. Antibody: Affinity purified rabbit anti-Mre11 (Cat. No. orb1523783) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human Mre11 (shaded) in Jurkat cells by flow cytometry.Antibody: Rabbit anti-Mre11 polyclonal antibody (orb1523783) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

Mre11

UniProt

P49959

RefSeq (Protein):NP_005582.1