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Rabbit Mouse IgG (H&L), ALP conjugated Antibody

SKU: orb699956

Description

Rabbit polyclonal antibody against Rabbit anti-Mouse IgG (H&L) conjugated to AP

Images & Validation

Tested ApplicationsELISA, ICC, IHC, WB
Dilution range1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
ReactivityMouse
Application Notes
Based on IEP, this antibody binds to: heavy chains on mouse IgGlight chains on all mouse immunoglobulinsNo reactivity is observed to non-immunoglobulin mouse serum proteins based in immunoelectrophoresis.

Key Properties

HostRabbit
ClonalityPolyclonal
ImmunogenPurified Mouse IgG, whole molecule,
PurificationImmunogen affinity purified rabbit IgG.
ConjugationALP

Storage & Handling

StorageNon-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Form/AppearanceLiquid, clear, colorless.
DisclaimerFor research use only

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
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ICC
Immunocytochemistry
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Rabbit Mouse IgG (H&L), ALP conjugated Antibody (orb699956)

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1 mg
$ 580.00
DispatchUsually dispatched within 5-10 working days
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