Rabbit METAP1 Antibody

SKU: orb1520809

Description

METAP1 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 20 µl/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 336 and C-terminus
• Target: METAP1
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

MAP 1; MAP1A; metAP 1; MetAP1A; methionine aminopeptidase 1; peptidase M 1

Detection of mouse METAP1 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METAP1 antibody (orb1520809) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human METAP1 by western blot. Samples: Whole cell lysate (10 µg) from Jurkat, HeLa, HEK293T, MCF-7, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-METAP1 antibody (orb1520809) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human METAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-METAP1 antibody (orb1520809) used for IP at 2 µg per reaction.

Quick Database Links

Gene Symbol

METAP1

UniProt

P53582

RefSeq (Protein):NP_055958.2