Rabbit Met Recombinant Monoclonal Antibody

SKU: orb1806454

Description

Rabbit Met Recombinant Monoclonal Antibody

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution Range: Application : Flow-Cyt , ApplicationDilutionRange : Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells. , Application : ICC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. , Application : IHC , ApplicationDilutionRange : 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. , Application : IP , ApplicationDilutionRange : 6 µl/mg lysate , Application : WB , ApplicationDilutionRange : 1:1000
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR283L
• Immunogen: Between 1340 and C-terminus
• Target: Met
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

AUTS9;c-Met;DFNB97;hepatocyte growth factor receptor;HGF receptor;HGF/SF receptor;HGFR;proto-oncogene c-Met;RCCP2;scatter factor receptor;SF receptor;tyrosine-protein kinase Met

Detection of human MET by western blot. Samples: Whole cell lysate (10 µg) from GaMG, T-47D, HT-29, OVCAR-8, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human MET by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human MET by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human MET (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human MET by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from GaMG cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MET recombinant monoclonal antibody (orb1806454) used for IP at 6 µl/mg lysate.

Detection of human MET by immunocytochemistry. Sample: FFPE section of GaMG cells. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human MET by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

Met

UniProt

P08581

RefSeq (Protein):NP_000236.2