Rabbit MED13L Antibody

SKU: orb1526658

Description

MED13L Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 to 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 550 and 600
• Target: MED13L
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mediator complex subunit 13-like; mediator of RNA polymerase II transcription subunit 13-like; MRFACD; PROSIT240; THRAP2; thyroid hormone receptor-associated protein 2; thyroid hormone receptor-associated protein complex 240 kDa component-like; TRAP240L

Detection of human MED13L by western blot. Samples: Whole cell lysate (15 µg) from HeLa, GaMG, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-MED13L antibody orb1526658 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human MED13L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-MED13L antibody orb1526658 used for IP at 6 µg per reaction.

Detection of human MED13L by immunohistochemistry.Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-MED13L used at 1: 1, 000 (0.2µg/ml).Detection: DAB

Quick Database Links

Gene Symbol

MED13L

UniProt

Q71F56

RefSeq (Protein):NP_056150.1