Rabbit anti-MCM2 Antibody

• Catalog Number: orb1530570
• Category: Antibodies
• Description: MCM2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MCM2
• UniProt ID: P49736
• NCBI: NP_0045117.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: D3S3194; MITOTIN; minichromosome maintenance prote
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human MCM2 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, and K-562 cells. Antibody: Affinity purified rabbit anti-MCM2 antibody at 0.1 µg/ml.

Detection of mouse MCM2 by western blot.Samples: Whole cell lysate from 70Z/3, CH27, CTLL-2, EL4, and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-MCM2 antibody at 0.1 µg/ml.

Detection of human MCM2 by immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-MCM2 used at a dilution of 1:5, 000 (0.2µg/ml).

Detection of human MCM2 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-MCM2 antibody.