Rabbit anti-MARCKS-like1 Antibody

• Catalog Number: orb1526738
• Category: Antibodies
• Description: MARCKS-like1 Antibody
• Target: MARCKS-like1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 145 and C-terminus
• UniProt ID: P49006
• Tested applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 5 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: macrophage myristoylated alanine-rich C kinase sub
• Note: For research use only
• NCBI: NP_075385.1

Detection of human MARCKS-like 1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MARCKS-like 1 antibody orb1526738 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 10 µg/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 15 seconds (B).

Detection of human MARCKS-like 1 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MARCKS-like 1 (Cat. No. orb1526738) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB