Rabbit MafA Recombinant Monoclonal Antibody

SKU: orb1519835

Description

MafA Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: WB - 1:1000; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Zinc-fixative (JB fix) is recommended to enhance staining.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Zinc-fixative (JB fix) is recommended to enhance staining.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: BLR067G
• Immunogen: Between 125 and 175
• Target: MafA
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

hMafA; INSDM; pancreatic beta-cell-specific transcriptional activator; RIPE3b1; RIPE3b1 factor; transcription factor MafA; transcription factor RIPE3b1; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A

Detection of mouse MafA by western blot. Samples: Whole cell lysate (10 µg) from Beta-TC-6, AlphaTC1 Clone 9, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human MafA by western blot. Samples: Whole cell lysate (10 µg) from HEK293T transfected with myc tagged Human MafA, Human MafB, and Human cMaf prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit recombinant monoclonal antibody to Myc-tag.

Detection of mouse MafA by western blot. Samples: Whole cell lysate (10 µg) from HEK293T transfected with myc tagged mouse MafA, Human MafB, and Human cMaf prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit recombinant monoclonal antibody to Myc-tag.

Detection of mouse MafA by immunohistochemistry. Sample: FFPE section of mouse pancreas (JB fixation). Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human MafA by immunohistochemistry. Sample: FFPE section of human pancreatic tumor. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse MafA by immunohistochemistry. Sample: FFPE section of mouseBeta-TC-6 cells. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human MafA (red) by immunohistochemistry. Sample: FFPE section of human pancreatic carcinoma. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (orb1519835) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

MafA

UniProt

Q8NHW3

RefSeq (Protein):NP_919331.1