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Description
Research Area
Images & Validation
−| Tested Applications | IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:5,000 - 1:15,000; IP - 1 - 4 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1 and 50 |
| Target | MAD1 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Detection of human MAD1 by WB and IP.Samples: HeLa cells. Antibody: Affinity purified rabbit anti-MAD1 antibody orb1530339 used at 0.1 µg/ml for WB (A) and at 1 µg/mg lysate for IP (B).

Detection of human MAD1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAD1 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human MAD1 by western blot.Samples: Whole cell lysate from HeLa cells. Antibody: Affinity purified rabbit anti-MAD1 antibody orb1530339 at 0.06 µg/ml.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_003541.2 |
|---|
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Protocol Information
Rabbit MAD1 Antibody (orb1530339)
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