Rabbit anti-MAD1 Antibody

• Catalog Number: orb1530339
• Category: Antibodies
• Description: MAD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:5,000 - 1:15,000; IP - 1 - 4 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MAD1
• UniProt ID: Q9Y6D9
• NCBI: NP_003541.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: MAD1; mitotic-arrest deficient 1, yeast, homolog-l
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human MAD1 by WB and IP.Samples: HeLa cells. Antibody: Affinity purified rabbit anti-MAD1 antibody orb1530339 used at 0.1 µg/ml for WB (A) and at 1 µg/mg lysate for IP (B).

Detection of human MAD1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAD1 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human MAD1 by western blot.Samples: Whole cell lysate from HeLa cells. Antibody: Affinity purified rabbit anti-MAD1 antibody orb1530339 at 0.06 µg/ml.