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Catalog Number | orb1710818 |
---|---|
Category | Antibodies |
Description | LOX-1 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | BLR262L |
Tested applications | ICC, IHC, WB |
Reactivity | Human |
Immunogen | Residues 61-273 (ECD) |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB: 1:1,000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | LOX-1 |
UniProt ID | P78380 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
Alternative names | LOX1; ox LDL receptor 1; SLOX1; scavenger receptor Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human LOX-1 by western blot. Samples: Whole cell lysate (25 µg) from DLD-1, MCF-7, and SiHa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710818) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human LOX-1 by immunohistochemistry. Sample: FFPE section of human gastric carcinoma. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710818). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human LOX-1 by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710818). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human LOX-1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710818). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human LOX-1 by immunocytochemistry. Sample: FFPE section of human DLD-1 cells. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710818). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human LOX-1 by immunocytochemistry. Sample: FFPE section of human NCI-H226 cells. Antibody: Rabbit anti-LOX-1 recombinant monoclonal antibody (orb1710818). Secondary: HRP-conjugated goat anti-rabbit IgG.
ICC, IHC, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |